Ge protein purification manual

Recombinant Protein Purification Handbook Principles and Methods Protein Purification Handbook Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods 2-D Electrophoresis using immobilized pH gradients Principles and Methods Microcarrier Cell Culture Principles and Methods.  · purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the. target protein from the affinity tag. Each intein tag contains a chitin binding domain (CBD) for the affinity purification of . For a background on techniques for protein purification, in general, and handling recombinant proteins, we recommend the Recombinant Protein Purification Handbook and other handbooks in this series (see Related literature on page ). Challenging proteins.

RTS Application Manual 5 Protein purification Purification of a His6-tagged Green Fluorescent Protein (GFP) Principle You can add either a N- or C-terminal His 6-tag to the protein that you want to express if you use the RTS pIVEX His 6-tag 2 nd generation vector set (pIVEXd; pIVEXd, see. GE Healthcare HisTrap HP, 1 ml and 5 ml HisTrap™ HP is a ready to use column, prepacked with precharged Ni Sepharose™ High Performance. This prepacked column is ideal for preparative purification of Histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). Ge healthcare protein purification manual GE Healthcare Bio-Sciences AB Björkgatan 30 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5, D Freiburg, Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire, HP7 9NA UK GE Healthcare Bio-Sciences Corp Centennial Avenue P.O. Box Piscataway, NJ USA GE Healthcare.

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